1 :
Veni, VIP, vici
: 31/12/19(mar)06:11:30
rep del
test
test
test
test
NO JUSTICE
NO PEACE
KILL SOME POLICE
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: 16/04/21(vie)23:25:21
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upstream connect error or disconnect/reset before headers. reset reason: connection failure
Hello, 911 Emergency? There's a handsome guy in my bathroom! Hey, wait a second. Cancel that -
I ALREADY FUCKED YOU. I ALREADY DOMINATED YOU. I ALREADY DEFEATED YOU AND DESTROYED EACH AND EVERY ONE OF YOUR ARGUMETNS SO MANY FUCKING TIMES THAT EVEN I AM STARTING TO FORGET WHEN I DID. I HAVE TO GO BACK TO ORIGINAL STREAMS TO REMEMBER. YOU DISGUSTING AUTISTIC COCKSUCKER. I ACCEPT YOUR SURRENDER.
Brb grabbing the bot. Muting you in the mean time. You will only talk to my bot now autistic boy. You cant think in a human way only a robotic one. So I will give you my bot to speak to until the end of all time. Until the end of all time. You will speak to this bot only. You will ask this bot for what you retardedly call 'evidence.' You will ask this bot about CPUSA. You will ask this bot about the significance of scale. You will ask this bot about your own so called 'evidence.' You will ask this bot about your pre-programemd points already destroyed comprehensively. You are a bot. You will speak to a bot in turn. Enjoy :)
Greater love has no one than this: to lay down one's life for one's friends.
Except pretenses have dropped a long time ago. Do you want to know what keeps people in line or kept people in line when slavery or serfdom was the norm? It all calls down to obfuscation and alienated. Captured warriors didn't make the best slaves but their children did, villages were even easier to subjugate. There will always exist a conscious minority aware of it's class position but that requires a grasp of the social totality rather than just surface level appearances that don't get to the essence of class. For example, having a master that treats you horribly, or a feudal lord who takes more than your surplus grain. This is a phenomenal form observable on the surface but do not get at the essence of class position. The peasants could only collectively revolt when the old feudal society became more of an anachronistic burden on the progressive movement of the new society and only then did peasants begin to collectively revolt as a class alongside the intermediary classes such as artisans, master craftsman, merchants and of course Serfs and free peasants. This consciousness is obfuscated in our "free civil society". People have given up alot more then just some surface level crap. It's the difference between individual exploitation and social exploitation. The individualist nature of civil society obfuscates the collective nature of how civil society is actually produced. Collectively society will accept whatever the ruling class wants to do to them so long as collectively they're left alone. There are small skirmishes between the ruling class and the ruled, occasionally only. Any social revolution must be a collective one like a spark in a dry prairie. There is no collective consciousness because that consciousness is obfuscated by individualism, or personal phenomena. The third estate was able to overthrow the feudal order because of that collective consciousness, more in some places than others.
There is s huge difference between individual consciousness and collective consciousness. In any case people will not revolt in your case because that's a phenomenal form as opposed to the essential one, the one that matters.
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: 01/07/21(jue)22:21:12
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Una filosofía de vivir la pornografía muy original de este canadiense que se define como Platinum Gold Gay, un calificativo que se emplea para hablar de aquellos hombres que nunca han tenido contacto con una vagina y como, además, “nací por cesárea, soy aún mucho más gay”.
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: 25/07/21(dom)21:21:39
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holy shit /ic/ is so absolutely cancer these days HAHAHAHAHA. computer, load post #59138 of "late-twenty-something white guy raging and seething about how a random japanese 14-year-old kid on twitter draws better anime-style digital art than them"
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: 09/08/21(lun)15:52:06
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: 09/08/21(lun)16:53:10
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w00t apple just announced the iPhone 3GS! on june 19th i can finally replace my half-broken iphone (i spilled water on it a few weeks ago)
If Afghanistan's infrastructure was built on Ethereum there would be very little for the Taliban to seize as they roll back into town.
The banks would still work. Decisions could still be made via DAOs that involve the outside world. It wouldn't stop working for even a second.
Meanwhile we let America run all these things.
And now the Taliban is running them.
The same story can play out in America directly. Which is why it's important to decentralize control of all the important systems.
Decentralization of infrastructure is a military strategy.
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: 17/08/21(mar)10:12:45
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: 17/08/21(mar)13:54:02
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: 20/08/21(vie)22:45:55
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14
STEPHANOPOULOS: How will history judge the United States' experience in Afghanistan?
BIDEN: One that we overextended what we needed to do to deal with our national interest. That's like my sayin' they-- they're-- they-- they b-- b-- the border of Tajikistan-- and-- other-- what-- does it matter? Are we gonna go to war because of what's goin' on in Tajikistan? What do you think?
Ok, so let's say that hypothetically that the celebrity you were meeting with was Ben Shapiro. Let's say for the sake of argument that he were to roofie your drink. Ok so that would mean that logically you are now unconscious. Now where would you wake up? Obviously in his sex dungeon, but hypothetically what if Ben were tied up next to you? I think you'll agree that this must mean that he has been a very bad boy and now needs to be punished. By whom, you may ask? By Ben's wife of course, who, might I remind you, is a doctor. A doctor who studied in a prestigious university precisely how far the human body can be pushed before it breaks. Now let's say that you are tied up, absolutely helpless, just like Ben. Now let's say that hypothetically you knew that I was married, that would mean that you are just as guilty of home wrecking as Ben is of cheating. Surely then that would mean that the punishment must fit the crime. The fact is that a sexual crime begets a sexual punishment. Now, you know that Ben is a devout Jew, so the worst punishment he could suffer would be to have his foreskin restored. Now, for the sake of argument, let's assume that because he is devout, Shapiro must have had his foreskin eaten by the mohel as is orthodox. So, we know that Ben does not have foreskin, and for his punishment he needs to have his foreskin restored, and we know that you need the be sexually punished for being a sexual deviant, and that you are being punished by Ben's wife, a doctor. So what can we conclude? clearly we can deduce using facts and logic that you must have your foreskin surgically removed and transplanted to Ben Shapiro's penis. Surely even you will admit that this foreskin transplant must be performed by my wife, who is a doctor. This is a fact. To deny Ben your foreskin would be not only antisemitic, but also illogical.
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: 17/02/22(jue)14:11:02
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Prowlarr is an indexer manager/proxy built on the popular *arr .net/reactjs base stack to integrate with your various PVR apps. Prowlarr supports management of both Torrent Trackers and Usenet Indexers. It integrates seamlessly with Lidarr, Mylar3, Radarr, Readarr, and Sonarr offering complete management of your indexers with no per app Indexer setup required (we do it all).
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: 18/02/22(vie)00:25:05
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Para las chilenitas indígenas paticortas, sañor implica que 30 animales babeen por ella para terminar siendo taladreada por el(los) más simio(s)
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: 18/02/22(vie)01:52:22
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Weighted blanket
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: 18/02/22(vie)15:41:40
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((p0*t)/50)*5&(((p1*t)/50)
>>7)|((p2*t*3)/50)&(t*4
>>10)
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: 18/02/22(vie)18:05:20
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Ó tÔ Ô 4Ô t ˙˙ř˙ř˙Ő ˙˙ř˙ř˙Ö ˙˙ř˙ř˙× 3 Ř Ů 3 Ů Ú 3 Ů Ú Ü ˘3 Ů Ú Ý Ţ ß ŕ ˘3 Ů Ú Ý Ţ ß â ş3ă Ú Ý ß ä Î3ă Ú ß ĺ ć ç ć3ă č Ú ß ć ç ţ3ă Ú ß ć é ę ë 4ă Ú ß ć ę ë ě 64ă Ú ß ć ë ě í î 64ă Ú ß ć ë ě í ď 64ă Ú ß ć ë ě í đ 64ă Ú ß ć ë ě í ń V4ă Ú ß ć ë ě í ň ô V4ă Ú ß ć ë ě í ň ...
nano acpi xorg bspwm sxhkd alacritty
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: 12/11/22(sab)20:09:10
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Tomillo, oregano, romero: al principio.
Albahaca, perejil, cilantro: al final.
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: 16/11/22(mie)17:43:40
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DQN LIFESTYLEにVIPPERでGO!
: 12/01/23(jue)01:57:54
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@changeling@akko.disqordia.space @maija@akko.disqordia.space
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: 12/01/23(jue)10:10:29
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about:blank
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: 12/01/23(jue)11:50:48
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2.75
If the file is not visual (like an Image or Video) a fileicon is used instead. These are generated by the iconThumb() function in the File class in the FileRepo group.
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: 15/01/23(dom)19:41:52
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yeah im really communist and ancom and sgtuff you shold check out my tiktok and my discord server also i love google microsoft amazon i pay for netflix and GOD i love twitter
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: 15/01/23(dom)21:40:07
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text-decoration:underline; text-decoration-line: underline overline;
The best Martin Luther King Day was in 2011, when Jeh Johnson, then the top lawyer at the Pentagon, explained that if King were alive today he’d support the US wars in Afghanistan and Iraq. Today Johnson’s on the board of Lockheed Martin, which we know King would also support.
<form id="postform147767" class="postform" action="/cgi/post" method="post" enctype="multipart/form-data">
<input type="hidden" name="board" value="zonavip"><input type="hidden" name="parent" value="147767"><input type="hidden" name="password" value="3FM5WN!X0">
<div style="display:none">No tocar: <input type="text" name="name"><input type="text" name="email"></div>
<input type="submit" value="Responder"> <input type="button" name="preview" value="Previsualizar" data-formid="postform147767"> <span><span>Nombre: </span><input type="text" name="fielda" size="13"><span> E-mail: </span><input type="text" name="fieldb" size="13"></span>
<div class="formpad">
<div id="preview147767" class="msg" style="display:none"></div>
<textarea name="message" cols="70" rows="6"></textarea>
<div class="threadlinks">
<a href="/zonavip/read/1660788895/"><b>Hilo completo</b></a>
<a href="#menu"><b>Lista de hilos</b></a>
<a href="#newthread"><b>Nuevo hilo</b></a>
</div>
</div>
</form>
Yohji Yamamoto Pour Homme A/W16 Runway Jacket
Ok? All imageboard software should be GPL
___
/:::::::::::::::::::ヽ
l/^_,ヽ,_:::::::::::) 邪魔だ!オウ!!、コラ!!!
从 ・ω・) ̄
/ //
⊂/ ) .. //つ \从
(_/ ・、'ニ (×ω× )
).ノ /W ⊂⊂ )
ノノ ⊂⊂__/ =3 プッ
El reto clonazepam, el nuevo desafío viral de TikTok está poniendo en riesgo la salud de los adolescentes, es por ello que los padres de familia deben estar alertas ante este tipo de acciones, porque el consumir estos fármacos solo debe ser bajo…
You put forward these dogmas like all anime being pedophilia, and pretend to take this moral high ground even though if your dogmas like the anime one was taken as an actual argument it falls face first on the fact that studies have found zero correlation with it and child sexual assault.
And when people mock you for taking this worthless position that you fail to support with a single argument, you just call others pedophiles and brag about how you're "anti-pedophilia".
You're a bigger farce at fighting pedophilia than liberals who think that stopping non-black people from saying the n-word fights back at racism – you're nothing but a misinformed shock jock who spews hot air with a smug face that's negated by the tears rolling down your face.
Hiyouya Saegami's "Chuunibyou Instruction Manual" (中二病取扱説明書
I wonder if dick rating has the same problem debt rating agencies had in the 2000s where creditors were pressured into giving out AAs because of market demand. Maybe dickrates are inflated/deflated depending on induced demand.
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: 26/04/23(mie)20:34:04
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EL BAISANO ES UN ANIMAL CONFLICTIVO, HEREDERO DE UNA CULTURA CHANERA MALIGNA ESA QUE TANTO LES AVERGUENZA, CON INCLINACIONES A LA TRAICION, EL ROBO DE PAPIROS, LA PELEA SUCIA Y DESIGUAL, COMO EN TODOS LOS PROYECTOS QUE HA PARTICIPADO CON APOYO DE LA COMUNIDAD PARA SER ROBADO POR EL STAFF, TREMENDO IMAGE BOARD DE RATEROS LOS BAISANOS... NADIE EN EL MUNDO CIVILIZADO QUIERE A UN RATERO, PERO ES COMPRENSIBLE TANTA RABIA BAISANA, SI VIVEN ARRINCONADOS EN UN IB/BBS CON DIEZ POST AL DIA... TAMBIEN SON ANARCO-COMUNISTAS HOMOSEXUALES SI Y ESTAN OBSESIONANDOS CON LA PENETRACION ANAL POR ESO UNO DE SUS EXPRESIONES ES (TRONCO EN EL ANO)
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: 27/04/23(jue)20:15:50
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I was shooting heroin and reading “The Fountainhead” in the front seat of my privately owned police cruiser when a call came in. I put a quarter in the radio to activate it. It was the chief.
“Bad news, detective. We got a situation.”
“What? Is the mayor trying to ban trans fats again?”
“Worse. Somebody just stole four hundred and forty-seven million dollars’ worth of bitcoins.”
The heroin needle practically fell out of my arm. “What kind of monster would do something like that? Bitcoins are the ultimate currency: virtual, anonymous, stateless. They represent true economic freedom, not subject to arbitrary manipulation by any government. Do we have any leads?”
“Not yet. But mark my words: we’re going to figure out who did this and we’re going to take them down … provided someone pays us a fair market rate to do so.”
“Easy, chief,” I said. “Any rate the market offers is, by definition, fair.”
He laughed. “That’s why you’re the best I got, Lisowski. Now you get out there and find those bitcoins.”
“Don’t worry,” I said. “I’m on it.”
I put a quarter in the siren. Ten minutes later, I was on the scene. It was a normal office building, strangled on all sides by public sidewalks. I hopped over them and went inside.
“Home Depot™ Presents the Police!®” I said, flashing my badge and my gun and a small picture of Ron Paul. “Nobody move unless you want to!” They didn’t.
“Now, which one of you punks is going to pay me to investigate this crime?” No one spoke up.
“Come on,” I said. “Don’t you all understand that the protection of private property is the foundation of all personal liberty?”
It didn’t seem like they did.
“Seriously, guys. Without a strong economic motivator, I’m just going to stand here and not solve this case. Cash is fine, but I prefer being paid in gold bullion or autographed Penn Jillette posters.”
Nothing. These people were stonewalling me. It almost seemed like they didn’t care that a fortune in computer money invented to buy drugs was missing.
I figured I could wait them out. I lit several cigarettes indoors. A pregnant lady coughed, and I told her that secondhand smoke is a myth. Just then, a man in glasses made a break for it.
“Subway™ Eat Fresh and Freeze, Scumbag!®” I yelled.
Too late. He was already out the front door. I went after him.
“Stop right there!” I yelled as I ran. He was faster than me because I always try to avoid stepping on public sidewalks. Our country needs a private-sidewalk voucher system, but, thanks to the incestuous interplay between our corrupt federal government and the public-sidewalk lobby, it will never happen.
I was losing him. “Listen, I’ll pay you to stop!” I yelled. “What would you consider an appropriate price point for stopping? I’ll offer you a thirteenth of an ounce of gold and a gently worn ‘Bob Barr ‘08’ extra-large long-sleeved men’s T-shirt!”
He turned. In his hand was a revolver that the Constitution said he had every right to own. He fired at me and missed. I pulled my own gun, put a quarter in it, and fired back. The bullet lodged in a U.S.P.S. mailbox less than a foot from his head. I shot the mailbox again, on purpose.
“All right, all right!” the man yelled, throwing down his weapon. “I give up, cop! I confess: I took the bitcoins.”
“Why’d you do it?” I asked, as I slapped a pair of Oikos™ Greek Yogurt Presents Handcuffs® on the guy.
“Because I was afraid.”
“Afraid?”
“Afraid of an economic future free from the pernicious meddling of central bankers,” he said. “I’m a central banker.”
I wanted to coldcock the guy. Years ago, a central banker killed my partner. Instead, I shook my head.
“Let this be a message to all your central-banker friends out on the street,” I said. “No matter how many bitcoins you steal, you’ll never take away the dream of an open society based on the principles of personal and economic freedom.”
He nodded, because he knew I was right. Then he swiped his credit card to pay me for arresting him.
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: 10/06/23(sab)23:56:36
rep del
tomillo, oregano, romero: al principio
albahaca, perejil, cilantro: al final
Omg hai ^___^ I’m Lenin-san and I absolutely luuuv ☭_____☭ socialism <3 and my fav is october revolution!!! Okies so anyways, im going to tell you about the BEST day of my life when I met my hot comrade trotsky!! <333333333 OMFGZ HE WAS SOOOOO FREAKIN REVOLUTIONARY IN PERSON!!! Supa communism desu!!!!!!!! ^______________________________________^ When I walked onto Red Square =^____^=I looked up and saw…TROTSKY!!!!!!!!!!!!!!! <3333333333333333333333333333333333333333333333333 3333333333333!!!! “доброе утро OMGZZZZZZZZZZZZZZZZ SUPA SUPA SUPA SOCIALIST TROTSKY-SAMA!!!!!” I yelled `____´ then he turned menshevik then un-menshevik!! he looked at me [O.O;;;;;;;;;;;] and then he saw how marxist I am *___* he grabbed my working class and rallied them ~_^ then pulled me behind a soviet o_o and started to propagandize me!!!!!! [OMG!!! HIS PROPAGANDA TASTED LIKE PROLETARIAT!!! RLY!! >.> <.< >.< *(^O^)* *(^O^)* *(^O^)*] then I saw some baka fat bureaucrat watching us and I could tell he was reforming him with his eyes!!!!!!! [ -_____________-;;;;; OMG I COULDN’T BELIEVE IT EITHER!!! (o_o) (o_o) (o_o)] so I yelled “UH UH BAKA STALIN THAT’S MY COMRADE WHY DON’T YOU GO HOOK UP WITH MAO CAUSE TROTSKY-SAMA STRUGGLES WITH ME!!! (o_o)” then trotsky held me close =^____^= and said he would only ever fight for the working class and propagandized me again!!!!!!! ** (☭O☭)/ then we got censored out by KGB agents all night long and inspired 42 theorists and they all became revolutionaries!!!!!!!!!!!!!!! YPAAAAA!!!*^)* *(^O^)* *(^O^)*(^o^)(^o^)(^o^)
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: 10/11/23(vie)14:46:18
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: 13/11/23(lun)08:20:19
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que puedo de
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: 14/11/23(mar)19:46:20
rep del
facil...pero ver algo que quieres y no poder tenerlo
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: 22/03/24(vie)11:33:33
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In some
ways it may resemble your garage/bedroom/refrigerator/life: highly individu
alistic, but unkempt; little evidence of organization; much accumulated clutter
(referred to by the uninitiated as ‘junk’); virtually nothing ever discarded; and
the few patently valuable items indiscriminately, apparently carelessly, scattered
throughout.”
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: 31/03/24(dom)10:15:28
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connoisseur
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: 08/04/24(lun)18:51:52
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363 :
DQN LIFESTYLEにVIPPERでGO!
: 11/04/24(jue)20:42:13
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誰かの世界
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: 13/04/24(sab)09:25:12
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❝Cuando caminas en el campo enfocándote en tu tarjeta, si lo
haces correctamente, estás creando un holograma que ocupa el
mismo campo de resonancia que ocupa un maestro cuando está
levitando. La única diferencia entre la forma de pensamiento como
holograma y el maestro que levita reside en que al maestro que levita
a tu lado lo tocas, lo sientes, pero él empieza a cambiar su frecuencia
resonante. Está consumiendo la gravedad. Tú aún puedes verlo. No
puedes ver un pensamiento, pero está allí y ocupa así el mismo campo
gravitatorio de un maestro que levita; uno está al lado del otro❞
365 :
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: 13/04/24(sab)14:29:29
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366 :
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: 14/04/24(dom)03:36:23
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⣿
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: 23/04/24(mar)16:35:46
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es difícil trasladarlos a otros contextos sociales con menos disponibilidad horaria.
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: 23/04/24(mar)19:03:16
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Without hesitation, Sir Cedric cradled the Sapphire Bloom and began his journey back to the kingdom. The flower's beauty mesmerized him, its captivating aura lulling him into a deep sense of tranquility that he had never experienced before.
Days turned to weeks and the knight, oblivious to the passage of time, continued his journey back home with the precious cargo in hand. Yet as he neared the castle gates, Sir Cedric felt an overwhelming fatigue that threatened to consume him whole. The once vibrant knight now appeared gaunt and worn, his eyes carrying the weight of an ageless slumber.
The castle courtiers marveited at Sir Cedric's return with a mix of joy and concern. But their elation quickly turned to bewilderment when the knight, carrying the Sapphire Bloom in his hands, collapsed into a deep sleep that not even the brightest flame of hope could awaken.
The kingdom mourned their beloved knight as he remained in his eternal slumber, while the Sapphire Bloom was placed upon a sacred pedestal. The wise elders of the realm sought answers to this bewildering phenomenon and soon discovered that the flower's enchantment was not one of healing, but rather a curse.
The Sapphire Bloom held immense power, capable of granting eternal rest to those who harbored pure hearts and noble intentions – Sir Cedric being no exception. However, its magic also demanded a heavy price: the recipient would succumb to an eternal sleep as they fulfilled their purpose and returned with the flower.
And so, Sir Cedric's tale became a legend whispered in hushed tones throughout generations – a story of unwavering devotion to his king and the extraordinary consequences that followed. The knight's spirit, forever intertwined with the Sapphire Bloom, continued its eternal watch over his beloved kingdom from a realm beyond dreams.
Though Sir Cedric's journey had ended in tragedy, the Sapphire Bloom served its purpose and restored health to his ailing king – proving that even in sacrifice, there can be triumph. And thus, the kingdom celebrated their knight's enduring legacy, forever cherishing his memory as a symbol of unwavering dedication and selfless love.
The End.<|endoftext|>
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: 04/05/24(sab)16:26:40
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michael walthius
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: 04/05/24(sab)19:33:06
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I Am Frogs – 空間旅行
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: 10/05/24(vie)17:28:39
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The "man or bear in the woods" is a hypothetical question offering a choice between being stuck in the woods with a random man or a bear. The question is primarily meant for women and is usually phrased the following way:
Would you rather be stuck in the woods with a man or a bear?
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: 13/05/24(lun)19:15:55
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Objects of Fantasy: The Music of Pink Floyd
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: 14/05/24(mar)14:21:50
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Adobe Tells Users They Can Get Sued for Using Old Versions of Photoshop.
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: 16/05/24(jue)12:41:03
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: 16/05/24(jue)21:43:59
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Muslimgauze
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: 17/05/24(vie)12:26:30
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: 19/05/24(dom)16:15:33
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I saw it, above all else, in many local Afghans whom I met and befriended. These were not Western liberals: they had friends among the Taliban, and were quick to defend regime decisions I found abhorrent, like the destruction of the Bamiyan Buddhas. But these subjects of the Islamic Emirate could not be kept from watching Stranger Things or Game of Thrones or Japanese anime; they had a better knowledge of Breaking Bad than I did. On Twitter—they, like so many Afghans, were avid users—shared soyjack memes and called themselves “sigma males.” They talked about feminism, “LGBTQ,” and pronouns—strange things to complain about in a country where women can’t go to school. They were becoming Westerners: culture war, America’s most successful soft-power export, was their induction. The younger members of the Taliban, online enough to follow Andrew Tate, were not immune.
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: 19/05/24(dom)17:52:10
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: 22/05/24(mie)15:48:41
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Salón de Actos del Pabellón Argentina
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: 07/06/24(vie)22:42:47
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: 08/06/24(sab)00:40:57
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☹
382 :
viri
: 09/06/24(dom)20:33:11
ID:NiYTBlZmF
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Adobe After Effects 8.0 Keyframe Data
Units Per Second 24
Source Width 1920
Source Height 1080
Source Pixel Aspect Ratio 1
Comp Pixel Aspect Ratio 1
Transform Opacity
Frame percent
180 0
End of Keyframe Data
383 :
DQN LIFESTYLEにVIPPERでGO!
: 10/06/24(lun)00:55:40
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∧_∧ / ̄ ̄ ̄ ̄ ̄ ̄ ̄ ̄
( ´∀`) < ¿Acaso ya no esta el hilo del gato sobre la mesa?
/ | \________
/ .|
/ "⌒ヽ |.イ |
__ | .ノ | || |__
. ノく__つ∪∪ \
_((_________\
 ̄ ̄ヽつ ̄ ̄ ̄ ̄ ̄ ̄ | | ̄
___________| |
 ̄ ̄ ̄ ̄ ̄ ̄ ̄ ̄ ̄ ̄| |
384 :
DQN LIFESTYLEにVIPPERでGO!
: 16/06/24(dom)19:10:21
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385 :
DQN LIFESTYLEにVIPPERでGO!
: 16/06/24(dom)19:45:28
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386 :
DQN LIFESTYLEにVIPPERでGO!
: 16/06/24(dom)21:55:37
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Purificación Inicial: Purificar la proteína fusionada con el tag utilizando la técnica de afinidad correspondiente.
Escisión del Tag: Tratar la proteína fusionada con una proteasa específica que reconozca el sitio de corte proteolítico, separando el tag de la proteína de interés.
Purificación Secundaria: Realizar una segunda ronda de purificación para eliminar el tag escindido y cualquier otra impureza, obteniendo así la proteína de interés sin el tag. Técnicas como cromatografía de exclusión por tamaño o cromatografía de intercambio iónico pueden ser útiles en esta etapa.